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KMID : 0613820140240080851
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Journal of Life Science
2014 Volume.24 No. 8 p.851 ~ p.859
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Medicinal Herb Extracts Attenuate 1-Chloro-2,4dinitrobenzene-induced Development of Atopic Dermatitis-like Skin Lesions
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Lee Moon-Hee
Han Min-Ho
Yoon Jung-Jeh
Song Myung-Kyu
Kim Min-Ju
Hong Su-Hyun
Choi Byung-Tae
Kim Byung-Woo
Hwang Hye-Jin
Choi Yung-Hyun
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Abstract
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The present study was designed to investigate whether ethanol extracts of Sophora flavescens (GS), Glycyrrhiza uralensis (GC), Dictamnus dasycarpus (BSP), and their mixtures (GGB-1, -2, -3, and -4) inhibit 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD) in a mouse model. DNCB was topically applied on the dorsal surface of Balb/c mice to induce AD-like skin lesions. The pathological phenotypes of AD, such as erythema, ear thickness, edema, scabs, and discharge, were significantly decreased in the GGB (DNCB + GS:GC:BSP = 3:1:1 mixture)-1-treated groups compared with the other treated groups. The weight of the spleen in immune organs was significantly decreased in the GGB-1-treated groups, whereas the weight of the liver in a control group was similar to that of the groups treated with the samples. Furthermore, toluidine blue staining analysis, a method used to specifically identify mast cells, showed that master cell infiltration into the dermis of the GGB-1-treated group was significantly decreased. The immunoglobulin E concentration was lower in the GGB-1- treated group. In addition, the levels of inflammatory cytokines (interferon-γ, interleukin-1, 4, 5, 6, and 13, 1β, and tumor necrosis factor-α) were also significantly reduced in the GGB-1-treated group. Taken together, these results suggest that a mixture of GS, GC, and BSP in a proportion of 3:1:1 (GGB-1) may contribute to the relief of AD symptoms and may be considered an excellent candidate for an AD therapeutic drug.
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KEYWORD
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Atopic dermatitis, cytokine, IgE, mast cell, medicinal herbal extract
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